WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification
  • WarmStart® feature inhibits activity at room temperature
  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 


Ouça uma entrevista com a Profa. Mayana Zatz sobre este tipo de teste aqui no Jornal da Usp: LINK
Decodificando o DNA
A coluna Decodificando o DNA, com a professora Mayana Zatz, vai ao ar quinzenalmente toda quarta-feira às 10h50, na Rádio USP (São Paulo 93,7 FM; Ribeirão Preto 107,9 FM) e também no Youtube, com produção do Jornal da USP e TV USP.