WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification
  • WarmStart® feature inhibits activity at room temperature
  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication



Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 

 

 

 

 

 

WarmStart Colorimetric LAMP 2X Master Mix (DNA&RNA)

WarmStart Colorimetric LAMP 2X Master Mix (DNA&RNA)

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification
  • WarmStart® feature inhibits activity at room temperature
  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication



Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.