T4 DNA Ligase

  • Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
  • This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
  • T4 DNA ligase will seal nicks for these DNA substrates.
  • The industry standard for performance and quality
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart


  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer

Product Source

Purified from E. coli C600 pcl857 pPLc28 lig8 (2).

Reagents Supplied

The following reagents are supplied with this product:

Properties and Usage

Unit Definition

One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.

400,000 cohesive end units/ml and 2,000,000 cohesive end units/ml

Reaction Conditions

1X T4 DNA Ligase Reaction Buffer
Incubate at 16°C

1X T4 DNA Ligase Reaction Buffer
50 mM Tris-HCl
10 mM MgCl2
1 mM ATP
10 mM DTT
(pH 7.5 @ 25°C)

Storage Temperature -20°C

Storage Conditions

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation - 65°C for 10 min