Fidelity at its finest.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
Reagents Supplied
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| Q5® Reaction Buffer Pack | -20 | 5 X |
| Q5® High GC Enhancer | -20 | 5 X |
Códigos
| Catalog # | Concentration | Size | |||
|---|---|---|---|---|---|
| M0491S | 2000 units/ml | 100 units | |||
| M0491L | 2000 units/ml | 500 units |
| M0492S | 2 X | 100 reactions (50 µl vol) |
| M0492L | 2 X | 500 reactions (50 µl vol) |
| M0493S | 2000 units/ml | 100 units |
| M0493L | 2000 units/ml | 500 units |
| M0494S | 2 X | 100 rxn (2 x 1.25 ml) |
| M0494X | 2 X | 500 rxn (1 x 12.5 ml) |
| M0494L | 2 X | 500 rxn (10 x 1.25 ml) |
