Fidelity at its finest.

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. 

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.

Product Source

An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.

Reagents Supplied

The following reagents are supplied with this product:

  Store at (°C) Concentration
Q5® Reaction Buffer Pack -20 5 X
Q5® High GC Enhancer -20 5 X


Catalog # Concentration Size      
M0491S 2000 units/ml 100 units
M0491L 2000 units/ml 500 units
M0492S 2 X 100 reactions (50 µl vol)
M0492L 2 X 500 reactions (50 µl vol)
M0493S 2000 units/ml 100 units
M0493L 2000 units/ml 500 units
M0494S 2 X 100 rxn (2 x 1.25 ml)
M0494X 2 X 500 rxn (1 x 12.5 ml)
M0494L 2 X 500 rxn (10 x 1.25 ml)