With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.
WHAT THE LUNA-FL™ DOES BEST
TWO COUNTERS IN ONE
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date.
COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells.
GFP TRANSFECTION ANALYSIS
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
THE SOFTWARE THAT CHANGES EVERYTHING.
FOUR COUNTING MODES
THE LOWEST COST. THE GREATEST CONVENIENCE.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images. Hong D, Lee G, Jung NC, Jeon M. Biological procedures online. 15(1):13.(2013) Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors. Ramirez K, Sheridan JF. Brain, behavior, and immunity. 57:293-303 (2016) High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1;78(9):1405-1411. (2016) Minicirclemicroporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials. 101:310-320 (2016) Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of pharmacology and experimental therapeutics. 358(3):457-63. (2016) Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts. Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Journal of medical virology. 88:1408-1416(2016) Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of surgical research. 204:361-370 (2016) In vitro endothelialization test of biomaterials using immortalized endothelial cells. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS one. 11:e0158289 (2016) High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific reports. 6:27301 (2016) Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International journal of oncology. 48(5):2205-12. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature medicine. 22:547-556 (2016) Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform. Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Nucleic acids research. 44:e88 (2016) Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone. Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Stem Cells 34(4):888-901 (2016) The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Nagai K, Yanagawa Y, Katagiri S, Nagano M. Biomedical research. 37:63-71 (2016) Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model. Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. J BioactCompatPolym 2016; doi: 10.1177/0883911516635841 Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells. Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Journal of natural products. 79:317-323 (2016) Application of a non-hazardous vital dye for cell counting with automated cell counters. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical biochemistry,1;492:8-12(2016) Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles. Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Reproductive biology and endocrinology : RB&E. 15;14:3 (2016) Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells. Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Plasma Med 2016; 10.1615/PlasmaMed.2016016219 GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations. Ramirez K, Niraula A, Sheridan JF. Brain, behavior, and immunity 51:154-68 (2016) Mouse model for sublethal Leptospira interrogans infection. Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. Infection and immunity. 83(12):4693-700.(2015) Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury. Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. J The Journal of surgical research. 199(1):56-66.2015; Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6. Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. International journal of oncology. 47(1):122-32 (2015) Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor. Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Molecules and cells. 38(6):548-61. (2015) Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Brain, behavior, and immunity. 46:212-20. Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones. Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Stem cells international. 2015:165867 (2015) β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. Experimental & molecular medicine. 47:e143. (2015)
LUNA-FL™: GFP Transfection Assay Quick Start Guide LUNA-FL™ Quick Start Guide LUNA-FL™ Brochure Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images Product literature Application of a non-hazardous vital dye for cell counting with automated cell counters GFP expression analysis using the LUNA-FL™ Fast cell counting and viability measurement of yeast cells with the LUNA-FL™ Automated fluorescence cell counting Logos Biosystems Products Brochure LUNA-FL™ software 3.0.1 Software Autoexposure mode update Notice
Compatible Reagents
WHAT DO OUR CUSTOMERS HAVE TO SAY?
Testimonials
Gelina Sani
Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
Mikayla Lopes
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
Regina Wulff, MS
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Danh Tran
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
Woong Sun, PhD
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
May Malicdan, MD PhD
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
Joanna Ireland, PhD
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
WHERE THE LUNA-FL™ HAS BEEN CITED
Citations
Thrombin preconditioning of extracellular vesicles derived from mesenchymal stem cells accelerates cutaneous wound healing by boosting their biogenesis and enriching cargo content. 2019. Sung DK, Chang YS, Sung SI, Ahn SY, Park WS. Journal of Clinical Medicine 8(4): 533.
Homotypic and heterotypic trans-assembly of human Rab-family small GTPases in reconstituted membrane tethering. 2019. Segawa K, Tamura N, Mima J. Journal of Biological Chemistry jbc.RA119.007947.
Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells. 2019. Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y. Scientific Reports 9(1): 3630.
Exploiting evolutionary herding to control drug resistance in cancer. 2019. Acar A, Nichol D, Fernandez-Mateos J, Cresswell GD, Barozzi I, Hong SP, Spiteri I, Stubbs M, Burke R, Stewart A, Vlachogiannis G, Maley CC, Magnani L, Valeri N, Banerji U, Sottoriva A. bioRxiv 566950.
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
HL-60 (high density)
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Jurkat
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Yeast Viability Kit 1 - Propidium Iodide Stain for Yeast (1 x 0.5 mL) - Fluorescein Diacetate Stain (1 x 0.5 mL) - Cell Dilution Buffer (1 x 20 mL) - Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
1 kit
F23212
Cell Dilution Buffer
5 x 20 mL
F23102
LUNA™ Fluorescence Calibration Beads
1 x 0.5 mL
B13101
LUNA™ Standard Beads
2 x 1 mL
F24003
LUNA-FL™ IQ/OQ Protocol
1 copy
P10001
LUNA™ Printer
1 unit
P10002
LUNA™ Printer Paper - thermal, 700 prints
3 x 2 rolls
LUNA-FL™ Dual Fluorescence (L20001) LogosBio
Esgotado
Compartilhar:
With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.
WHAT THE LUNA-FL™ DOES BEST
TWO COUNTERS IN ONE
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date.
COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells.
GFP TRANSFECTION ANALYSIS
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
THE SOFTWARE THAT CHANGES EVERYTHING.
FOUR COUNTING MODES
THE LOWEST COST. THE GREATEST CONVENIENCE.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images. Hong D, Lee G, Jung NC, Jeon M. Biological procedures online. 15(1):13.(2013) Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors. Ramirez K, Sheridan JF. Brain, behavior, and immunity. 57:293-303 (2016) High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1;78(9):1405-1411. (2016) Minicirclemicroporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials. 101:310-320 (2016) Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of pharmacology and experimental therapeutics. 358(3):457-63. (2016) Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts. Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Journal of medical virology. 88:1408-1416(2016) Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of surgical research. 204:361-370 (2016) In vitro endothelialization test of biomaterials using immortalized endothelial cells. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS one. 11:e0158289 (2016) High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific reports. 6:27301 (2016) Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International journal of oncology. 48(5):2205-12. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature medicine. 22:547-556 (2016) Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform. Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Nucleic acids research. 44:e88 (2016) Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone. Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Stem Cells 34(4):888-901 (2016) The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Nagai K, Yanagawa Y, Katagiri S, Nagano M. Biomedical research. 37:63-71 (2016) Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model. Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. J BioactCompatPolym 2016; doi: 10.1177/0883911516635841 Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells. Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Journal of natural products. 79:317-323 (2016) Application of a non-hazardous vital dye for cell counting with automated cell counters. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical biochemistry,1;492:8-12(2016) Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles. Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Reproductive biology and endocrinology : RB&E. 15;14:3 (2016) Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells. Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Plasma Med 2016; 10.1615/PlasmaMed.2016016219 GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations. Ramirez K, Niraula A, Sheridan JF. Brain, behavior, and immunity 51:154-68 (2016) Mouse model for sublethal Leptospira interrogans infection. Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. Infection and immunity. 83(12):4693-700.(2015) Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury. Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. J The Journal of surgical research. 199(1):56-66.2015; Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6. Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. International journal of oncology. 47(1):122-32 (2015) Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor. Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Molecules and cells. 38(6):548-61. (2015) Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Brain, behavior, and immunity. 46:212-20. Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones. Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Stem cells international. 2015:165867 (2015) β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. Experimental & molecular medicine. 47:e143. (2015)
LUNA-FL™: GFP Transfection Assay Quick Start Guide LUNA-FL™ Quick Start Guide LUNA-FL™ Brochure Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images Product literature Application of a non-hazardous vital dye for cell counting with automated cell counters GFP expression analysis using the LUNA-FL™ Fast cell counting and viability measurement of yeast cells with the LUNA-FL™ Automated fluorescence cell counting Logos Biosystems Products Brochure LUNA-FL™ software 3.0.1 Software Autoexposure mode update Notice
Compatible Reagents
WHAT DO OUR CUSTOMERS HAVE TO SAY?
Testimonials
Gelina Sani
Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
Mikayla Lopes
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
Regina Wulff, MS
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Danh Tran
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
Woong Sun, PhD
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
May Malicdan, MD PhD
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
Joanna Ireland, PhD
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
WHERE THE LUNA-FL™ HAS BEEN CITED
Citations
Thrombin preconditioning of extracellular vesicles derived from mesenchymal stem cells accelerates cutaneous wound healing by boosting their biogenesis and enriching cargo content. 2019. Sung DK, Chang YS, Sung SI, Ahn SY, Park WS. Journal of Clinical Medicine 8(4): 533.
Homotypic and heterotypic trans-assembly of human Rab-family small GTPases in reconstituted membrane tethering. 2019. Segawa K, Tamura N, Mima J. Journal of Biological Chemistry jbc.RA119.007947.
Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells. 2019. Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y. Scientific Reports 9(1): 3630.
Exploiting evolutionary herding to control drug resistance in cancer. 2019. Acar A, Nichol D, Fernandez-Mateos J, Cresswell GD, Barozzi I, Hong SP, Spiteri I, Stubbs M, Burke R, Stewart A, Vlachogiannis G, Maley CC, Magnani L, Valeri N, Banerji U, Sottoriva A. bioRxiv 566950.
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
HL-60 (high density)
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Jurkat
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
