Reagent |
jetOPTIMUS® DNA Transfection Reagent |
---|---|
Molecule delivered |
Plasmid DNA |
Applications |
Transient and stable gene expression from plasmid DNA transfection |
Cell types |
Adherent hard-to-transfect mammalian cell: |
Number of transfections |
1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
Storage |
5°C ± 3°C, stable for at least one year when stored appropriately |
Provided with |
jetOPTIMUS® Buffer |
Ace your DNA transfection in the cell model you need!
As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
Reference number | Reagent size | Buffer size |
---|---|---|
117-01 | 0.1 mL | 10 mL |
117-07 | 0.75 mL | 2 x 60 mL |
117-15 | 1.5 mL | 4 x 60 mL |
717-60 | x | 60 mL |
Bulk quantities are available upon request. Please contact us.
jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible, and reducing the volume of reagent required compared to main competitors. Consequently, scientists can increase the number of transfections and reduce the cost per reaction by up to seven-fold (table 1).
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
If you have any questions regarding jetOPTIMUS® please visit our dedicated Frequently asked questions or contact us at support@polyplus-transfection.com.