The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores; see the fluorophore compatibility chart
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • Sample Trays and Smart Tray Technology™ — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

For western blotting, the ChemiDoc™ MP V3 Western Workflow™ provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab™ Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi ChemiDoc MP V3 Western Workflows also include:

  • ChemiDoc MP Imaging System
  • Mini-PROTEAN® Tetra Cell, 4-gel (1658004) or Criterion™ Cell (1656001) Electrophoresis Chamber
  • PowerPac™ Basic Power Supply (1645050)
  • Trans-Blot® Turbo™ Transfer System (1704150)
  • 4–20% Mini-PROTEAN® TGX Stain-Free™ or 4–20% Criterion™ TGX Stain-Free™ Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot® LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR® Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Product Catalog Number
ChemiDoc MP Imaging System 17001402
ChemiDoc MP V3 Western Workflow for Mini Gels 17001395
ChemiDoc MP V3 Western Workflow for Midi Gels 17001389

Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc V3 Western Workflows for Mini Gels (17001403), and ChemiDoc V3 Western Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

Related Products

Fluorophore reference poster for western blotting and gel imaging

Request a Free Fluorophore Reference Poster
Determine the best fluorophore for maximum sensitivity and high signal-to-noise ratio in your next experiment using this poster.

Related Categories

Automation Capabilities  
Smart Tray Technology™ Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus Precalibrated focus for any zoom setting or sample height
Auto-exposure Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding Dynamic; precalibrated and optimized for each application
Touch-screen functionality Multitouch capability (2 points) 12.1" display
Maximum image area (W x H) 21 x 16.8 cm
Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination sources Trans-UV, 302 nm excitation
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Filter and application 590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output 16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight 35 kg (78 lb)
Instrument size (L x W x H) 61 x 51 x 53 cm
Operating voltage 100–250 V
Operating temperature 10–28°C
Operating humidity

10–85% relative humidity (non-condensing)


Number Description Options
7051 Imaging Systems Family Flier   [ Add to Cart (Free) ]
6873 ChemiDoc™ MP Imaging System Brochure, Ver B    
6936 ChemiDoc MP Imaging System Flier, Ver A    
10000062126 ChemiDoc™ and ChemiDoc MP Imaging Systems with Image Lab™ Touch Software User Guide    
6427 Bio-Rad® V3 Western Workflow™, Ver D   [ Add to Cart (Free) ]
10000060300 Acquiring an Image with ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver A    
10000060301 Acquiring a Multichannel Image with the ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver B    
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide   [ Add to Cart (Free) ]