ChemiDoc™ MP Imaging System
The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.
Features and Benefits
- All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
- Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores; see the fluorophore compatibility chart
- Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
- Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
- Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
- Sample Trays and Smart Tray Technology™ — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
- Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.
For western blotting, the ChemiDoc™ MP V3 Western Workflow™ provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.
Applications and Uses
- Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
- Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection
Included Components
- Internal computer with Image Lab™ Touch Software
- 12” touch screen display
- Image Lab Software to install on your computer
- Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)
Mini and Midi ChemiDoc MP V3 Western Workflows also include:
- ChemiDoc MP Imaging System
- Mini-PROTEAN® Tetra Cell, 4-gel (1658004) or Criterion™ Cell (1656001) Electrophoresis Chamber
- PowerPac™ Basic Power Supply (1645050)
- Trans-Blot® Turbo™ Transfer System (1704150)
- 4–20% Mini-PROTEAN® TGX Stain-Free™ or 4–20% Criterion™ TGX Stain-Free™ Protein Gels
- Buffers, Nitrocellulose Transfer Packs, Immun-Blot® LF PVDF Membranes, and other consumables
Optional Components
- White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
- Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR® Stain (12003027)
- Holder for ChemiDoc Sample Trays and UV Shield (1708377)
- Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
- UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)
Configuration Options
| Product | Catalog Number |
| ChemiDoc MP Imaging System | 17001402 |
| ChemiDoc MP V3 Western Workflow for Mini Gels | 17001395 |
| ChemiDoc MP V3 Western Workflow for Midi Gels | 17001389 |
Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc V3 Western Workflows for Mini Gels (17001403), and ChemiDoc V3 Western Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).
Related Products
Request a Free Fluorophore Reference Poster
Determine the best fluorophore for maximum sensitivity and high signal-to-noise ratio in your next experiment using this poster.
- StarBright Blue 700 Fluorescent Secondary Antibodies
- hFAB™ Rhodamine Housekeeping Protein Fluorescent Primary Antibodies
- PrecisionAb™ Validated Western Blotting Antibodies
- Clarity and Clarity Max Western ECL Blotting Substrates
- Protein Stains
- Nucleic Acid Stains and Tracking Dyes
Related Categories
- ChemiDoc and ChemiDoc XRS+ Imaging Systems
- Protein Gels including TGX Stain-Free Gels
- Western Blotting
- Nucleic Acid Electrophoresis and Blotting
| Automation Capabilities | |
| Smart Tray Technology™ | Automatic recognition of application-specific tray and adjustment of imaging parameters and software options |
| Autofocus | Precalibrated focus for any zoom setting or sample height |
| Auto-exposure | Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications |
| Image flat fielding | Dynamic; precalibrated and optimized for each application |
| Hardware | |
| Touch-screen functionality | Multitouch capability (2 points) 12.1" display |
| Maximum image area (W x H) | 21 x 16.8 cm |
| Detector | Cooled CCD, 6 megapixels |
| Dynamic range | >4 orders of magnitude |
| Illumination sources | Trans-UV, 302 nm excitation Epi-white Trans-white (requires White Sample Tray) Trans-blue, 450−490 nm excitation (requires Blue Sample Tray) Epi-blue, 460−490 nm excitation Epi-green, 520−545 nm excitation Epi-red, 625−650 nm excitation Epi-far red, 650−675 nm excitation Epi-near IR, 755−777 nm excitation |
| Filter and application | 590/110 nm standard filter to perform protein and DNA gel and blot imaging Chemiluminescence filter 518–546 nm filter for blue-excitable fluorophores and stains 577–613 nm filter for green-excitable fluorophores and stains 675–725 nm for red-excitable fluorophores and stains 700–730 nm filter for far red-excitable fluorophores and stains 813–860 nm for near IR-excitable fluorophores and stains |
| Data output | 16-bit or 8-bit: SCN, TIFF, JPEG files |
| Instrument weight | 35 kg (78 lb) |
| Instrument size (L x W x H) | 61 x 51 x 53 cm |
| Operating voltage | 100–250 V |
| Operating temperature | 10–28°C |
| Operating humidity |
10–85% relative humidity (non-condensing) |
| Number | Description | Options | |
|---|---|---|---|
| 7051 | Imaging Systems Family Flier | [ Add to Cart (Free) ] | |
| 6873 | ChemiDoc™ MP Imaging System Brochure, Ver B | ||
| 6936 | ChemiDoc MP Imaging System Flier, Ver A | ||
| 10000062126 | ChemiDoc™ and ChemiDoc MP Imaging Systems with Image Lab™ Touch Software User Guide | ||
| 6427 | Bio-Rad® V3 Western Workflow™, Ver D | [ Add to Cart (Free) ] | |
| 10000060300 | Acquiring an Image with ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver A | ||
| 10000060301 | Acquiring a Multichannel Image with the ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver B | ||
| 6493 | Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide | [ Add to Cart (Free) ] | |