Highlights


  • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
  • Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
  • High Sensitivity: Increased detection limit of very low abundance organisms.

Description


The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
 

Kit Components


Cat # Name Size
R1100-50 DNA/RNA Shield 50 ml
W1001-30 DNase/RNase-Free Water 30 ml
D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
R1003-3-24 RNA Wash Buffer 24 ml
R1060-1-50 RNA Lysis Buffer 50 ml
R1060-2-25 RNA Prep Buffer 25 ml
C1001-50 Collection Tubes 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
E1010-1-4 DNA Digestion Buffer 4 mL
 
 

Technical Specifications


Equipment Microcentrifuge, vortex, cell disrupter (recommended)
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range Total RNA ≥17 nt
Yield 100 µg RNA (binding capacity), ≥50 µl (elution volume)
ZymoBIOMICS RNA Kit
ZymoBIOMICS RNA Kit

Highlights


  • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
  • Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
  • High Sensitivity: Increased detection limit of very low abundance organisms.

Description


The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
 

Kit Components


Cat # Name Size
R1100-50 DNA/RNA Shield 50 ml
W1001-30 DNase/RNase-Free Water 30 ml
D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
R1003-3-24 RNA Wash Buffer 24 ml
R1060-1-50 RNA Lysis Buffer 50 ml
R1060-2-25 RNA Prep Buffer 25 ml
C1001-50 Collection Tubes 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
E1010-1-4 DNA Digestion Buffer 4 mL
 
 

Technical Specifications


Equipment Microcentrifuge, vortex, cell disrupter (recommended)
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range Total RNA ≥17 nt
Yield 100 µg RNA (binding capacity), ≥50 µl (elution volume)