Highlights


  • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
  • Ultra-Pure: DNA and Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
  • High Sensitivity: Increased detection limit of very low abundance organisms.

Description


The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.
 

Technical Specifications


Equipment Microcentrifuge, vortex, cell disrupter (recommended)
Purity DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range DNA and Total RNA ≥17 nt
Yield 100 µg DNA/RNA (binding capacity), ≥50 µl (elution volume)

Kit Components


Cat # Name Size
R1100-50 DNA/RNA Shield 50 ml
W1001-30 DNase/RNase-Free Water 30 ml
D7001-1-50 DNA/RNA Lysis Buffer 50 ml
D7010-2-50 DNA/RNA Prep Buffer 50 ml
D7010-3-24 DNA/RNA Wash Buffer (Concentrate) 24 ml
C1001-50 Collection Tubes 50 Pack
C1006-50-F Spin-Away Filters 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
E1010-1-4 DNA Digestion Buffer 4 mL

 

ZymoBIOMICS DNA/RNA Kit
ZymoBIOMICS DNA/RNA Kit

Highlights


  • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
  • Ultra-Pure: DNA and Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
  • High Sensitivity: Increased detection limit of very low abundance organisms.

Description


The ZymoBIOMICS DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.
 

Technical Specifications


Equipment Microcentrifuge, vortex, cell disrupter (recommended)
Purity DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range DNA and Total RNA ≥17 nt
Yield 100 µg DNA/RNA (binding capacity), ≥50 µl (elution volume)

Kit Components


Cat # Name Size
R1100-50 DNA/RNA Shield 50 ml
W1001-30 DNase/RNase-Free Water 30 ml
D7001-1-50 DNA/RNA Lysis Buffer 50 ml
D7010-2-50 DNA/RNA Prep Buffer 50 ml
D7010-3-24 DNA/RNA Wash Buffer (Concentrate) 24 ml
C1001-50 Collection Tubes 50 Pack
C1006-50-F Spin-Away Filters 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
E1010-1-4 DNA Digestion Buffer 4 mL