ZymoBIOMICS DNA kits
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ZymoBIOMICS DNA Kits
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. These microbial DNA purification kits include the ZymoBIOMICS lysis system which eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. These microbial DNA purification kits are equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA from these microbial DNA purification kits is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
ZymoBIOMICS DNA Microprep Kit
| Cat # | Name | Size |
|---|---|---|
| D4301 | ZymoBIOMICS DNA Microprep Kit (Lysis Tubes) | 50 Preps |
| D4305 | ZymoBIOMICS DNA Microprep Kit (No Lysis Matrix) | 50 Preps |
ZymoBIOMICS DNA Miniprep Kit
| Cat # | Name | Size |
|---|---|---|
| D4300 | ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) | 50 Preps |
| D4300T | ZymoBIOMICS DNA Miniprep Kit | 5 Preps |
| D4304 | ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) | 50 Preps |
ZymoBIOMICS 96 MagBead Kit
| Cat # | Name | Size |
|---|---|---|
| D4302 | ZymoBIOMICS 96 MagBead Kit (Lysis Rack) | 2 x 96 Preps |
| D4306 | ZymoBIOMICS 96 MagBead Kit (No Lysis Matrix) | 2 x 96 Preps |
| D4308 | ZymoBIOMICS 96 MagBead Kit (Lysis Tubes) | 2 x 96 Preps |
ZymoBIOMICS 96 DNA Kit
| Cat # | Name | Size |
|---|---|---|
| D4303 | ZymoBIOMICS 96 DNA Kit (Lysis Rack) | 2 x 96 Preps |
| D4307 | ZymoBIOMICS 96 DNA Kit (No Lysis Matrix) | 2 x 96 Preps |
| D4309 | ZymoBIOMICS 96 DNA Kit (Lysis Tubes) | 2 x 96 Preps |
Highlights
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Technical Specifications
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
|---|---|
| Elution Volume | ≥ 50 µl DNA Elution Buffer or DNase free water |
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
| Processing Volume | Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells) |
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 25 µg total DNA can be eluted into ≥50 µl |
