Highlights


  • High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
  • Highest Yields: Consistently purify > 30% more cfDNA.
  • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.

Description


Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.

Technical Specifications


DNA Purity High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.
DNA Recovery Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.
DNA Storage Eluted DNA should be stored at ≤ -20ºC
Processing volume Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m
l Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml
Recovery Volume ≥ 35 µl of DNA Elution Buffer or DNase free water.
Required Equipment Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge.
Sample Sources Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).
Quick-cfDNA Serum & Plasma Kit
Quick-cfDNA Serum & Plasma Kit

Highlights


  • High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
  • Highest Yields: Consistently purify > 30% more cfDNA.
  • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.

Description


Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.

Technical Specifications


DNA Purity High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.
DNA Recovery Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.
DNA Storage Eluted DNA should be stored at ≤ -20ºC
Processing volume Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m
l Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml
Recovery Volume ≥ 35 µl of DNA Elution Buffer or DNase free water.
Required Equipment Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge.
Sample Sources Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).