High efficiency recovery of cell-free DNA from serum, plasma, CSF, and amniotic fluid.

Documentation:

  • High-quality DNA, including cell-free, is easily and robustly purified from up to 10 ml of serum, plasma, amniotic fluid or cerebrospinal fluid.
  • Zymo-Spin™ technology enables elution of DNA in as little as 35 μl and ensures it is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.

Quick-cfDNA™ Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin™ technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.

Specification

  • Size Range: ≥100 bp
  • Equipment: Microcentrifuge, heat block/bath (55ºC), vacuum/vacuum manifold or swinging bucket centrifuge.
  • Sample Storage: Eluted DNA should be stored at ≤ -20ºC.
  • Purity: Ready for all sensative downstream applications such as qPCR and Next-Generation Sequencing
  • Yield: 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.
  • Sample Source: Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).
  • Processing Volume:
    Plasma – Single centrifugation: up to 3 ml
    Plasma – Double centrifugation: up to 10 ml
    Serum – up to 10 ml
    Amniotic fluid – up to 1 ml
    Cerebrospinal fluid (CSF) – up to 1 ml
    Saliva – up to 1 ml
    Cell-free Saliva – up to 5 ml
  • Type: Total DNA including cfDNA (Cell -free DNA)
  • Applicable For: Cell-free DNA isolation including ccfDNA and ctDNA from tumor cells, necrotic cells, and apoptotic cells. Most commonly used with liquid biopsies for use in fetal DNA and cancer research.
Product Protocol SDS
Quick-cfDNA™ Serum & Plasma Kit Protocol SDS (MSDS)

 

D4076 - Quick-cfDNA Serum & Plasma Kit - 50 preps - Zymo
R$7.180,00 R$5.000,00
Frete grátis
Quantidade
D4076 - Quick-cfDNA Serum & Plasma Kit - 50 preps - Zymo R$5.000,00

High efficiency recovery of cell-free DNA from serum, plasma, CSF, and amniotic fluid.

Documentation:

  • High-quality DNA, including cell-free, is easily and robustly purified from up to 10 ml of serum, plasma, amniotic fluid or cerebrospinal fluid.
  • Zymo-Spin™ technology enables elution of DNA in as little as 35 μl and ensures it is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.

Quick-cfDNA™ Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin™ technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.

Specification

  • Size Range: ≥100 bp
  • Equipment: Microcentrifuge, heat block/bath (55ºC), vacuum/vacuum manifold or swinging bucket centrifuge.
  • Sample Storage: Eluted DNA should be stored at ≤ -20ºC.
  • Purity: Ready for all sensative downstream applications such as qPCR and Next-Generation Sequencing
  • Yield: 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor.
  • Sample Source: Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).
  • Processing Volume:
    Plasma – Single centrifugation: up to 3 ml
    Plasma – Double centrifugation: up to 10 ml
    Serum – up to 10 ml
    Amniotic fluid – up to 1 ml
    Cerebrospinal fluid (CSF) – up to 1 ml
    Saliva – up to 1 ml
    Cell-free Saliva – up to 5 ml
  • Type: Total DNA including cfDNA (Cell -free DNA)
  • Applicable For: Cell-free DNA isolation including ccfDNA and ctDNA from tumor cells, necrotic cells, and apoptotic cells. Most commonly used with liquid biopsies for use in fetal DNA and cancer research.
Product Protocol SDS
Quick-cfDNA™ Serum & Plasma Kit Protocol SDS (MSDS)