HostZERO Microbial DNA Kit (50 preps), D4310, Zymo, Room Temp & Dry Ice Items, -70C / RT

Highlights


  • Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
  • Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
  • Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.

Description


The HostZERO Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research’s non-biased purification technology, the HostZERO Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS non-biased DNA isolation technology for accurate data representation. Finally, the HostZERO Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the HostZERO Microbial DNA Kit produces the highest-quality data for microbial samples.

Technical Specifications


DNA Purity High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing.
DNA Recovery Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted.
Processing Time Bacterial DNA is isolated from a single sample in less than 90 minutes.
Required Equipment Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended).
Sample Input Up to 200 µl liquid sample.
Sample Type Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells.

Kit Components


Cat # Name Size
R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml
D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg
D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg
D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml
D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml
D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml
D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml
D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
D4302-5-50 ZymoBIOMICS DNase/RNase Free Water 50 ml
C1001-50 Collection Tubes 50 Pack
C1001-500 Collection Tubes 500 Pack
C1001-1000 Collection Tubes 1000 Pack
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
D4310-1-20 Host Depletion Solution 20 ml
D4310-2-5 Microbial Selection Buffer 5 ml
D4310-3-50 Microbial Selection Enzyme 50 µl
HostZERO Microbial DNA Kit
HostZERO Microbial DNA Kit

HostZERO Microbial DNA Kit (50 preps), D4310, Zymo, Room Temp & Dry Ice Items, -70C / RT

Highlights


  • Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
  • Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
  • Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.

Description


The HostZERO Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research’s non-biased purification technology, the HostZERO Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS non-biased DNA isolation technology for accurate data representation. Finally, the HostZERO Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the HostZERO Microbial DNA Kit produces the highest-quality data for microbial samples.

Technical Specifications


DNA Purity High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing.
DNA Recovery Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted.
Processing Time Bacterial DNA is isolated from a single sample in less than 90 minutes.
Required Equipment Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended).
Sample Input Up to 200 µl liquid sample.
Sample Type Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells.

Kit Components


Cat # Name Size
R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml
D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg
D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg
D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml
D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml
D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml
D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml
D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
D4302-5-50 ZymoBIOMICS DNase/RNase Free Water 50 ml
C1001-50 Collection Tubes 50 Pack
C1001-500 Collection Tubes 500 Pack
C1001-1000 Collection Tubes 1000 Pack
S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
D4310-1-20 Host Depletion Solution 20 ml
D4310-2-5 Microbial Selection Buffer 5 ml
D4310-3-50 Microbial Selection Enzyme 50 µl