Cadastre-se Login
Início / Biologia Molecular / Extração e Purificação / DNA /

Direct-zol DNA/RNA Miniprep

Direct-zol DNA/RNA Miniprep

Highlights

  • One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.

Description

The Direct-zol™ DNA/RNA kits provide an innovative method for the purification of DNA and total RNA from a variety of samples freshly lysed in TRIzol or similar, including animal cells, tissue, bacteria, yeast, plant, biological liquids and etc.

Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin™ Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post-purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.
 

Technical Specifications

Binding Capacity Specification 25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample)
Compatibility Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield™ (see Appendix, page 5)
Elution Volume ≥ 25 µl
Equipment Microcentrifuge
Purity DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR)
Sample Inactivation TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents.
Sample Type Specifications Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar.
Size Range up to and above 40 kb (DNA), ≥ 17 nt (RNA)
Direct-zol DNA/RNA Miniprep
Direct-Zol RNA/DNA, escolha a versão::
R2080T - Direct-zol DNA/RNA Miniprep - 10 preps
Direct-Zol RNA/DNA, escolha a versão:
R2080T - Direct-zol DNA/RNA Miniprep - 10 preps
R2080 - Direct-zol DNA/RNA Miniprep - 50 preps
R2081 - Direct-zol DNA/RNA Miniprep (Com TRI reagente) - 50 preps
Componentes do Direct-Zol RNA/DNA::
W1001-1 - DNase/RNase-Free Water - 1 ml
Componentes do Direct-Zol RNA/DNA:
W1001-1 - DNase/RNase-Free Water - 1 ml
W1001-4 - DNase/RNase-Free Water - 4 ml
R1003-3-12 - RNA Wash Buffer - 12 ml
R1060-2-25 - RNA Prep Buffer - 25 ml
C1005-50 - Zymo-Spin III Columns - 50 Pack
R2050-1-50 - TRI Reagent - 50 ml
R2080-1-24 - Direct-zol DNA Wash 1 (concentrate) - 50 preps
R2080-2-40 - Direct-zol DNA Wash 2 - 50 preps
R2080-3-4 - Direct-zol DNA Elution Buffer - 50 preps
C1078-50 - Zymo-Spin IICR Columns - 50 Pack
Direct-zol DNA/RNA Miniprep

Highlights

  • One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.

Description

The Direct-zol™ DNA/RNA kits provide an innovative method for the purification of DNA and total RNA from a variety of samples freshly lysed in TRIzol or similar, including animal cells, tissue, bacteria, yeast, plant, biological liquids and etc.

Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin™ Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post-purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.
 

Technical Specifications

Binding Capacity Specification 25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample)
Compatibility Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield™ (see Appendix, page 5)
Elution Volume ≥ 25 µl
Equipment Microcentrifuge
Purity DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR)
Sample Inactivation TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents.
Sample Type Specifications Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar.
Size Range up to and above 40 kb (DNA), ≥ 17 nt (RNA)