Direct-zol DNA/RNA Miniprep
Highlights
- One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
Description
Technical Specifications
Binding Capacity Specification | 25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample) |
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Compatibility | Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield™ (see Appendix, page 5) |
Elution Volume | ≥ 25 µl |
Equipment | Microcentrifuge |
Purity | DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR) |
Sample Inactivation | TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents. |
Sample Type Specifications | Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar. |
Size Range | up to and above 40 kb (DNA), ≥ 17 nt (RNA) |