Luna® Universal qPCR & RT-qPCR

Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Each Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with NEB’s Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna One-Step RT-qPCR products is a novel, engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

For two-step RT-qPCR, the LunaScript™ RT SuperMix Kit offers a fast (less than 15 min), robust, and sensitive option for cDNA synthesis upstream of your Luna qPCR experiment. The supermix contains a blue tracking dye allowing you to easily track your samples throughout the RT-qPCR workflow.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix and supermix formats with user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your One-Step RT-qPCR with Luna WarmStart® Reverse Transcriptase 

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq. Unique aptamer technology increases reaction specificity and robustness.

Find the right Luna product for your application

Luna Product Info Graphic

Enjoy the convenience & compatibility

  • The Luna reference dye supports universal instrument compatibility (High-ROX, Low-ROX, ROX-independent)
  enjoy the convenience
Typical Luna qPCR amplification plots using various real-time instruments. As shown, Luna’s universal reference dye is compatible with high-ROX, low-ROX and ROX-independent instruments. Default instrument view is shown.
  • Quickly set up reactions using convenient master mixes and user-friendly protocols
  • Prevent carryover contamination by pre-treating with Antarctic Thermolabile UDG (NEB #M0372)
  • Eliminate pipetting errors with non-interfering, visible tracking dye
Luna LP visible tracking dye
Luna® qPCR and RT-qPCR NEB
Luna® qPCR and RT-qPCR NEB

Luna® Universal qPCR & RT-qPCR

Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Each Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with NEB’s Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna One-Step RT-qPCR products is a novel, engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

For two-step RT-qPCR, the LunaScript™ RT SuperMix Kit offers a fast (less than 15 min), robust, and sensitive option for cDNA synthesis upstream of your Luna qPCR experiment. The supermix contains a blue tracking dye allowing you to easily track your samples throughout the RT-qPCR workflow.

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix and supermix formats with user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your One-Step RT-qPCR with Luna WarmStart® Reverse Transcriptase 

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq. Unique aptamer technology increases reaction specificity and robustness.

Find the right Luna product for your application

Luna Product Info Graphic

Enjoy the convenience & compatibility

  • The Luna reference dye supports universal instrument compatibility (High-ROX, Low-ROX, ROX-independent)
  enjoy the convenience
Typical Luna qPCR amplification plots using various real-time instruments. As shown, Luna’s universal reference dye is compatible with high-ROX, low-ROX and ROX-independent instruments. Default instrument view is shown.
  • Quickly set up reactions using convenient master mixes and user-friendly protocols
  • Prevent carryover contamination by pre-treating with Antarctic Thermolabile UDG (NEB #M0372)
  • Eliminate pipetting errors with non-interfering, visible tracking dye
Luna LP visible tracking dye