When using tissue-derived cells such as stem cells or the cells from the adipose stromal vascular fraction (SVF), knowing cell concentration and viability is crucial to ensure and control the quality of downstream procedures. Tissue-derived samples are often contaminated with red blood cells and noncellular debris, making cell counting a time-consuming, subjective, and error-prone process. The LUNA-STEM™ is equipped with dual fluorescence and brightfield optics to distinguish live and dead nucleated cells from non-nucleated cells. Samples are stained with nucleic acid dyes to determine the concentration and viability of mononucleated cells. Non-nucleated cells are distinguished from debris and also counted. In as little as 30 seconds, the LUNA-STEM™ reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability, and average cell size. A high-resolution image with live cells labeled with green circles and dead cells with red circles can be viewed to confirm data visually.

WHAT THE LUNA-STEM™ DOES BEST

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF count tissue-derived cells
    COUNT TISSUE-DERIVED CELLS

    Using dual fluorescence and brightfield optics, the LUNA-STEM™ can distinguish between cells and debris.

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF 30 seconds to results
    30 SECONDS TO RESULTS

    In as little as 30 seconds, the LUNA-STEM™ captures, merges, and analyzes images from three channels to produce cell count and viability data.

  • UNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF  nucleated and non-nucleated cell analysis
    NUCLEATED AND NON-NUCLEATED CELL ANALYSIS

    Nucleic stains make sure that nucleated cells are counted as live or dead cells whereas nonnucleated cells are counted as non-nucleated cells.

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF easy record keeping
    EASY RECORD KEEPING

    PDF reports complete with images and data are automatically generated and can be transferred to a PC or printed for your records.

THE SOFTWARE THAT CHANGES EVERYTHING.

VERIFY RESULTS IMMEDIATELY USE THE DILUTION CALCULATOR CUSTOMIZE & SAVE PROTOCOLS

VERIFY RESULTS IMMEDIATELY

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9):1623-1633.
L

WHAT DO OUR CUSTOMERS HAVE TO SAY?

Testimonials
  • Patrick Mallory, DO

    Mallory Family Wellness

    The LUNA-STEM unit has proven to be a very convenient and useful addition to our stem cell procedures. It provides a quicker and more accurate method of counting nucleated cells from both bone marrow and adipose tissue than the traditional manual method. The printed reports are clean and easy to import to the patient’s electronic medical record. The process is also not only quicker than manual cell counting but is also cleaner, avoiding the mess, hassle and expense of dyes and hemocytometer slides and covers. The training provided by the LUNA-STEM field scientist was thorough, allowing quick implementation of the process. I highly recommend this unit without reservation to any clinic or facility that provides stem cell treatments.

  • Ann Erickson, Senior Research Associate

    California Pacific Medical Center Research Institute

    After 25 years of being a proponent of manual counting I now find myself a LUNA-STEM convert.

  • Roger Hogue, MD

    Hogue Cosmetic Surgery

    As a Regenerative Medicine Specialist with experience in the isolation and deployment of stromal vascular fraction (SVF) containing adipocyte-derived stem cells (ADSCs) for the treatment of degenerative orthopedic conditions since 2011, I have been asked to share my thoughts about the LUNA-STEM that I use in my practice.
    During the initial years, I used a manual microscopy method using trypan blue staining to perform cell counts and viability assessments of isolated SVF specimens. During the past year, I purchased a LUNA-STEM that uses AO/PI staining. The LUNA-STEM has increased my time efficiency and accuracy in determining cell counts for nucleated and non-nucleated cells within the SVF specimen, as well as cell viability assessments. The interactive touch screen on the LUNA-STEM is easy to use.
    Manual image focusing on an SVF specimen placed on a counting slide, referred to as a PhotonSlide, is very straightforward. Data reports and captured images can quickly be saved as a PDF file on an inserted USB thumb drive. For chart documentation, the LUNA Printer is great for an immediate printout of counting results.
    I can honestly say that I will not be going back to my old trypan blue staining method. The LUNA-STEM universally does a better job at cell counting and viability than I can do.

  • Heather Main, PhD

    Genea Biocells

    We have two LUNA-STEM systems. We like it because it’s a plug and go, no startup or shut down maintenance and only a single step for fluorescence based cell count and viability analysis. For what it is, the price competitiveness of the LUNA-STEM system and consumables cannot be beaten.

  • Andrew C. Wesely, MD

    Sierra Stem Cell Institute

    At Sierra Stem Cell Institute, we provide regenerative injection treatments for orthopedic and other musculoskeletal conditions, utilizing PRP and stem cell preparations from bone marrow and lipoaspirate. Knowing the cell count, and viability of our preparations, at the point of care became very important. The measuring process also needed to be easy implement, simple to perform, and cost effective. The LUNA-STEM proved an excellent solution to our needs and performed beyond expectations. I highly recommend this technology to any clinic providing these services.

+ SHOW OUR CUSTOMERS

WHERE THE LUNA-STEM™ HAS BEEN CITED

Citations
  • Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.
    2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.

  • Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.
    2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.

  • Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials
    2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.

  • Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.
    2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.

  • The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.
    2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.

Resources

Downloads
  • Icon
    LUNA-STEM

    Brochure

    2018-04-26 | 704 KB
  • Icon
    Logos Biosystems Products

    Brochure

    2018-04-17 | 2.14 MB
  • Icon
    Using the LUNA Reusable Slide for accurate cell counting with automated cell counters

    Product literature

    2018-04-13 | 665.84 KB
  • Icon
    How to Count Cells An Overview of Cell Counting Methods

    Product literature

    2018-04-13 | 720.79 KB
  • Icon
    LUNA-STEM™ software ver. 1.4.0

    Software

    2018-04-16 | 2.5 MB

ORDERING INFORMATION

Cat # Product Qty
L30001 LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF 1 unit
L12005 PhotonSlide™, 50 Slides 1 box
L12006 PhotonSlide™, 500 Slides 10 boxes
L12007 PhotonSlide™, 1000 Slides 20 boxes
L12011 LUNA™ Reusable Slide 1 unit
L12012 LUNA™ Reusable Slides 2 units
L12014 LUNA™ Reusable Slide Coverslips 10 units
F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
F23002 Acridine Orange Stain 2 x 0.5 mL
F23003 Propidium Iodide Stain 2 x 0.5 mL
F23212 Cell Dilution Buffer 5 x 20 mL
F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
L34003 LUNA-STEM™ IQ/OQ Protocol 1 copy
P10001 LUNA™ Printer 1 unit
P10002 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls
LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF (L30001) LogosBio
LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF (L30001) LogosBio
When using tissue-derived cells such as stem cells or the cells from the adipose stromal vascular fraction (SVF), knowing cell concentration and viability is crucial to ensure and control the quality of downstream procedures. Tissue-derived samples are often contaminated with red blood cells and noncellular debris, making cell counting a time-consuming, subjective, and error-prone process. The LUNA-STEM™ is equipped with dual fluorescence and brightfield optics to distinguish live and dead nucleated cells from non-nucleated cells. Samples are stained with nucleic acid dyes to determine the concentration and viability of mononucleated cells. Non-nucleated cells are distinguished from debris and also counted. In as little as 30 seconds, the LUNA-STEM™ reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability, and average cell size. A high-resolution image with live cells labeled with green circles and dead cells with red circles can be viewed to confirm data visually.

WHAT THE LUNA-STEM™ DOES BEST

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF count tissue-derived cells
    COUNT TISSUE-DERIVED CELLS

    Using dual fluorescence and brightfield optics, the LUNA-STEM™ can distinguish between cells and debris.

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF 30 seconds to results
    30 SECONDS TO RESULTS

    In as little as 30 seconds, the LUNA-STEM™ captures, merges, and analyzes images from three channels to produce cell count and viability data.

  • UNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF  nucleated and non-nucleated cell analysis
    NUCLEATED AND NON-NUCLEATED CELL ANALYSIS

    Nucleic stains make sure that nucleated cells are counted as live or dead cells whereas nonnucleated cells are counted as non-nucleated cells.

  • LUNA-STEM Automated Fluorescence Cell Counter for Stem Cells and SVF easy record keeping
    EASY RECORD KEEPING

    PDF reports complete with images and data are automatically generated and can be transferred to a PC or printed for your records.

THE SOFTWARE THAT CHANGES EVERYTHING.

VERIFY RESULTS IMMEDIATELY USE THE DILUTION CALCULATOR CUSTOMIZE & SAVE PROTOCOLS

VERIFY RESULTS IMMEDIATELY

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9):1623-1633.
L

WHAT DO OUR CUSTOMERS HAVE TO SAY?

Testimonials
  • Patrick Mallory, DO

    Mallory Family Wellness

    The LUNA-STEM unit has proven to be a very convenient and useful addition to our stem cell procedures. It provides a quicker and more accurate method of counting nucleated cells from both bone marrow and adipose tissue than the traditional manual method. The printed reports are clean and easy to import to the patient’s electronic medical record. The process is also not only quicker than manual cell counting but is also cleaner, avoiding the mess, hassle and expense of dyes and hemocytometer slides and covers. The training provided by the LUNA-STEM field scientist was thorough, allowing quick implementation of the process. I highly recommend this unit without reservation to any clinic or facility that provides stem cell treatments.

  • Ann Erickson, Senior Research Associate

    California Pacific Medical Center Research Institute

    After 25 years of being a proponent of manual counting I now find myself a LUNA-STEM convert.

  • Roger Hogue, MD

    Hogue Cosmetic Surgery

    As a Regenerative Medicine Specialist with experience in the isolation and deployment of stromal vascular fraction (SVF) containing adipocyte-derived stem cells (ADSCs) for the treatment of degenerative orthopedic conditions since 2011, I have been asked to share my thoughts about the LUNA-STEM that I use in my practice.
    During the initial years, I used a manual microscopy method using trypan blue staining to perform cell counts and viability assessments of isolated SVF specimens. During the past year, I purchased a LUNA-STEM that uses AO/PI staining. The LUNA-STEM has increased my time efficiency and accuracy in determining cell counts for nucleated and non-nucleated cells within the SVF specimen, as well as cell viability assessments. The interactive touch screen on the LUNA-STEM is easy to use.
    Manual image focusing on an SVF specimen placed on a counting slide, referred to as a PhotonSlide, is very straightforward. Data reports and captured images can quickly be saved as a PDF file on an inserted USB thumb drive. For chart documentation, the LUNA Printer is great for an immediate printout of counting results.
    I can honestly say that I will not be going back to my old trypan blue staining method. The LUNA-STEM universally does a better job at cell counting and viability than I can do.

  • Heather Main, PhD

    Genea Biocells

    We have two LUNA-STEM systems. We like it because it’s a plug and go, no startup or shut down maintenance and only a single step for fluorescence based cell count and viability analysis. For what it is, the price competitiveness of the LUNA-STEM system and consumables cannot be beaten.

  • Andrew C. Wesely, MD

    Sierra Stem Cell Institute

    At Sierra Stem Cell Institute, we provide regenerative injection treatments for orthopedic and other musculoskeletal conditions, utilizing PRP and stem cell preparations from bone marrow and lipoaspirate. Knowing the cell count, and viability of our preparations, at the point of care became very important. The measuring process also needed to be easy implement, simple to perform, and cost effective. The LUNA-STEM proved an excellent solution to our needs and performed beyond expectations. I highly recommend this technology to any clinic providing these services.

+ SHOW OUR CUSTOMERS

WHERE THE LUNA-STEM™ HAS BEEN CITED

Citations
  • Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.
    2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.

  • Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.
    2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.

  • Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials
    2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.

  • Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.
    2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.

  • The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.
    2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.

Resources

Downloads
  • Icon
    LUNA-STEM

    Brochure

    2018-04-26 | 704 KB
  • Icon
    Logos Biosystems Products

    Brochure

    2018-04-17 | 2.14 MB
  • Icon
    Using the LUNA Reusable Slide for accurate cell counting with automated cell counters

    Product literature

    2018-04-13 | 665.84 KB
  • Icon
    How to Count Cells An Overview of Cell Counting Methods

    Product literature

    2018-04-13 | 720.79 KB
  • Icon
    LUNA-STEM™ software ver. 1.4.0

    Software

    2018-04-16 | 2.5 MB

ORDERING INFORMATION

Cat # Product Qty
L30001 LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF 1 unit
L12005 PhotonSlide™, 50 Slides 1 box
L12006 PhotonSlide™, 500 Slides 10 boxes
L12007 PhotonSlide™, 1000 Slides 20 boxes
L12011 LUNA™ Reusable Slide 1 unit
L12012 LUNA™ Reusable Slides 2 units
L12014 LUNA™ Reusable Slide Coverslips 10 units
F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
F23002 Acridine Orange Stain 2 x 0.5 mL
F23003 Propidium Iodide Stain 2 x 0.5 mL
F23212 Cell Dilution Buffer 5 x 20 mL
F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
L34003 LUNA-STEM™ IQ/OQ Protocol 1 copy
P10001 LUNA™ Printer 1 unit
P10002 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls